SCT085

BioTracker^™ MemBright 640 Live Cell Dye

• Synonym(s): Live cell imaging probe, Live cell membrane probe, far red
• UNSPSC Code: 41106514
• NACRES: NA.47

Properties

• mol wt: observed mol wt 1396.4 kDa
• Quality Level: 100
• packaging: vial of 50 μL
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell based assay: suitable; flow cytometry: suitable
• detection method: fluorometric
• shipped in: cooler
• storage temp.: −20°C

Description

General description

The BioTracker^™ Membright-640 live cell dye is a “turn-on” fluorescence probe emitting in the far red spectrum for the imaging of plasma membranes of live cells.

Probes specific for the plasma membrane are fundamental tools for marking cell boundaries. Membright probes use a unique mechanism, existing in a self-quenched nanoparticle state. On contact and propagation within the cell membrane, the probe becomes fluorogenic, demonstrating remarkably high signal-to-background ratio. These live cell membrane probes are compatible with two-photon imaging, superresolution imaging, and are suitable for use in live or fixed cells. Their exceptional photostability and lack of cytotoxicity make Membright probes optimal for long-term imaging studies. The key characteristics of these plasma membrane probes permit use in a broad spectrum of fluorescence microscopy applications, and in flow cytometry.

Spectral Properties
Fluorescence images obtained by λex = 640 nm and emission range of 640-800 nm.

Reference

Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614

Application

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.

Features and Benefits

Far red fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.

Physical form

Blue solution

Storage and Stability

Store long term at -20°C, protected from light.

Other Notes

Live cell fluorescent imaging, flow cytometry

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup