MABS436

Anti-ATE1 Antibody, clone 6F11

clone 6F11, from rat

• Synonym(s): Arginyl-tRNA--protein transferase 1, Arginyltransferase 1, R-transferase 1, Arginine-tRNA--protein transferase 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6F11, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_038827
• UniProt accession no.: Q9Z2A5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ATE1(11101)

Description

General description

ATE1 plays an important role a specialized ubiquitin mediated protein degradation pathway. ATE1 is involved in the post-translational conjugation of arginine to the N-terminal or mid-chain Aspartate or Glutamate amino acids of proteins. The arginylation is required for effective ubiquitin mediated protein degradation. ATE1 is a central player in the Arg/N-end rule pathway of ubiquitin mediated protein degradation. This system disposes of malformed or misdirected proteins and protein fragments. ATE1 has two isoforms, isoform 1 is nuclear localized and isoform 2 is cytoplasmically localized. ATE1 is widely expressed, and recent research demonstrates that ATE1 mediated arginylation is essential for platelet myosin phosphorylation, clot retraction and thrombosis formation. Recent research also shows that the Arg/N-end rule pathway and ATE1 may play a role in helping to prevent neurodegeneration caused by the buildup of protein fragments found in various neurological disease states such as Alzheimer′s disease, amyotrophic lateral sclerosis, and Parkinson′s disease.

Immunogen

Recombinant protein corresponding to mouse ATE1.

Application

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-ATE1 Antibody, clone 6F11 is validated for use in Western Blotting and Immunocytochemistry for the detection of ATE1.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected ATE1 in 10 µg of mouse liver tissue lysate.
Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate.

Target description

~60 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable