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MABS1158

Sigma-Aldrich

Anti-PTPRT Antibody, clone 1F7

clone 1F7, from mouse

Synonym(s):

Receptor-type tyrosine-protein phosphatase T, R-PTP-T, RPTP-rho, RPTPrho, PTPRT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1F7, monoclonal

species reactivity

mouse, human, rat

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTPRT(11122)

General description

Receptor-type tyrosine-protein phosphatase T (EC 3.1.3.48; UniProt O14522; also known as R-PTP-T, Receptor protein tyrosine phosphatase, Receptor-type tyrosine-protein phosphatase rho, RPTP-rho, RPTPrho) is encoded by the PTPRT (also known as KIAA0283) gene (Gene ID 11122) in human. RPTPrho/PTPRT is a member of the R2B subfamily of receptor-type protein tyrosine phosphatases (R-PTPs). Known RPTPrho/PTPRT substrates include BCR (Tyr177), paxillin (Tyr188), and STAT3 (Tyr705). In addition, in vitro pull-downs and cell-free dephosphorylation assays also suggest cadherin and catenin family of cell adhesion molecules as physiological substrates of RPTPrho/PTPRT. PTPRT is the single most commonly mutated PTPR gene in all sequenced human cancers, with the highest PTPRT mutation frequency found in cutaneous melanoma. Among 16 tumor types examined, 37.9% of PTPRT mutations are found in the catalytic (PTPase) domain and 33.0% in the extracellular fibronectin type III-like (FN3) domain. In head and neck squamous cell carcinoma (HNSCC), 45.5% PTPRT mutations are located in the PTPase domain, leading to up-regulated STAT3 phosphorylation in HNSCC tumors.

Specificity

Clone 1F7 reacts with the full-length PTPRT as well as cleaved/truncated PTPRT forms that contain the two phosphatase domains.

Immunogen

Epitope: PTPase domains 1/2
GST-tagged recombinant protein corresponding to the PTPase domains 1/2 of human PTPRT.

Application

Anti-PTPRT Antibody, clone 1F7 is an antibody against PTPRT for use in Western Blotting.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Western Blotting Analysis: 1.0 µg/mL of this antibody detected the full-length and cleaved forms of PTPRT in rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected the full-length and a cleaved form of PTPRT in brain and liver homogenates from wild-type, but not PTPRT-knockout mice (Courtesy of Dr. Zhenghe Wang, Case Western Reserve University, Cleveland, OH).
Western Blotting Analysis: 2.0 µg/mL of this antibody detected the full-length and a cleaved form of PTPRT in small intestine epithelium homogenate from wild-type mice (Courtesy of Dr. Zhenghe Wang, Case Western Reserve University, Cleveland, OH).
Western Blotting Analysis: A representative lot detected exogenously expressed human PTPRT using transiently transfected HNCC CAL-33 (squamous carcinomas of the tongue) cells expressing wild-type, PTPase domain mutatant (A1022E), or FN3-domain mutatant (P497T) PTPRT, as well as in transfected HNSCC PCI-52-SD1 cells stably expressing wild-type human PTPRT (Lui, V.W., et al. (2014). Proc. Natl. Acad. Sci. U.S.A. 111(3):1114-1119).

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected the full-length and a cleaved form of PTPRT in mouse brain tissue lysate.

Target description

~ 190/90 kDa observed. Due to glycosylation, the full-length target band (~190 kDa) appears larger than the calculated molecular weight (162.1 kDa). Cleaved/truncated forms between ~70 kDa to 120 kDa can also be detected. It is believed that PTPRT is proteolytically cleaved upon activation. The intracellular portion containing the two protein tyrosine phosphatase (PTPase) domains then translocates into nucleus, where it dephosphorylates STAT3 (Zhang, X., et al. (2007). Proc. Natl. Acad. Sci. U.S.A. 104(10):4060-4064).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Frequent mutation of receptor protein tyrosine phosphatases provides a mechanism for STAT3 hyperactivation in head and neck cancer.
Lui, VW; Peyser, ND; Ng, PK; Hritz, J; Zeng, Y; Lu, Y; Li, H; Wang, L; Gilbert et al.
Proceedings of the National Academy of Sciences of the USA null

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