MABF3166

Anti-HCV E2 Antibody, clone AR2A

• Synonym(s): Genome polyprotein;Envelope glycoprotein E2
• UNSPSC Code: 12352203

Properties

• biological source: human
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: AR2A, monoclonal
• mol wt: calculated mol wt 327.15 kDa
• purified by: using protein G
• species reactivity: virus
• packaging: antibody small pack of 100 μL
• technique(s): ELISA: suitable; neutralization: suitable
• isotype: IgG1
• epitope sequence: N-terminal half
• Protein ID accession no.: N/A
• UniProt accession no.: P27958
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified
• Gene Information: vaccinia virus ... E2(684)

Description

General description

Genome polyprotein (UniProt: P27958) is encoded by the POLY gene (Gene ID: 951475) in Hepatitis C virus. HCV is a small (~55-65 nm), enveloped, positive-sense single-stranded RNA virus that is a causative factor for hepatitis C and hepatocellular carcinoma. HCV expresses several proteins that promote viral replication. Genome polyprotein of HCV can be cleaved into 11 different chains. The mature core protein packages viral RNA to form a viral nucleocapsid and promotes virion budding. It also prevents the establishment of cellular antiviral state by blocking the interferon- /β and interferon- signaling pathways and by inducing STAT1 degradation. Envelope proteins E1 and E2 that form a heterodimer are heavily glycosylated and are involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. In HCV, Viroprotein P7, a heptameric ion channel protein, plays an essential role in the assembly, envelopment, and secretion of viral particles. It also contains NS2, a cysteine protease and NS3, a serine protease. NS2 is required for the proteolytic auto-cleavage between NS2 and NS3. NS3 binds a single ATP and catalyzes the unzipping of a single base pair of dsRNA. Non-structural protein 4A (NS4A), a peptide cofactor, forms a non-covalent complex with the N-terminal of NS3 and this complex prevents phosphorylation of host IRF3, which also prevents the establishment of dsRNA induced antiviral state. NS4B induces a specific membrane alteration that serves as a scaffold for the virus replication complex. Non-structural protein 5A (NS5A), a multi-functional phosphoprotein, is an RNA-binding peripheral membrane protein that is reported to antagonize numerous cellular pathways, including the antiviral interferon-a response. NS5A exists as hypo- and hyper-phosphorylated forms and the dynamic transition between these two states is involved in the functions of NS5A. Hyperphosphorylation occurs primarily at its six serine residues within the low complexity sequence of NS5A. Phosphorylated NS5A is shown to be essential for viral replication and assembly. Clone AR2A detects a discontinuous epitope and specifically detects envelope protein E2. It does not block binding of E1E2 to CD81. (Ref.: Kong, L., et al. (2013). Science. 342(6162); 1090-1094; Giang, E., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(16); 6205-6210).

Specificity

Clone AR2A is a human recombinant antibody that detects envelope glycoprotein E2 of Hepatitis C virus. It recognizes a discontinuous epitope from the N-terminal half.

Immunogen

From a Fab phage-display library generated from a donor chronically infected with Hepatitis C virus (HCV).

Application

Quality Control Testing

Evaluated by ELISA in lysate from HEK293T cells transfected with H77E1E2.

ELISA Analysis (ELISA): Envelope glycoprotein E2 was detected by ELISA in various dilutions of lysates from HEK293T cells transfected with H77E1E2, but not in lysate from untransfected cells.

Tested Applications

ELISA Analysis: A representative lot detected HCV E2 in ELISA applications (Law, M., et al. (2008). Nat Med. 14(1):25-27; Giang, E., et al. (2012). Proc Natl Acad Sci. USA. 109(16):6205-10).

Neutralizing: A representative lot of this antibody neutralized several HCV isolates (Giang, E., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(16):6205-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Physical form

Purified human recombinant monoclonal antibody IgG1 in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable