MABF3054

Anti-SARS CoV-2 RBD mutant N501Y Antibody, clone 8A6

• Synonym(s): Spike glycoprotein Mutant N501Y
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 8A6, monoclonal
• mol wt: calculated mol wt 141 kDa
• purified by: using protein G
• species reactivity: vaccinia virus
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: N/A
• UniProt accession no.: P0DTC2
• storage temp.: 2-8°C
• Gene Information: vaccinia virus ... S(43740568)

Description

Specificity

Clone 8A6 is a mouse monoclonal antibody that detects N501Y mutant form of of SARS-CoV-2 spike protein.

Immunogen

KLH-conjugated linear peptide segment surrounding N501Y mutant site.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV-2 Spike RBD (N501Y) protein .

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant SARS-CoV-2 RBD mutant N501Y protein.

Tested Applications

ELISA Analysis: Serial dilutions of this antibody detected various recombinant SARS-CoV-2 protein samples

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Over the course of the pandemic, SARS-CoV2 has consistently mutated giving rise to various mutants. Clone 8A6 recognizes SARS-CoV-2 spike protein with N501Y mutation. Several variants, including B.2.2.7, B.1.351, and P.1 share this mutation on the receptor binding domain (RBD) and is a more infectious variant. RBD with N501Y mutation exhibits a stronger interaction with ACE2 receptor and slower dissociation rate. (Ref.: Liu, Y., et al. (2022). Nature. 602(7696); 294-299; Tian, F., et al. (2021). eLife. 10; e69091).

Physical form

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable