MABF149D

Anti-CD117 (c-Kit) (mouse), PE-Cy7, clone ACK2 Antibody

clone ACK2, from rat, PE-Cy7

• Synonym(s): Mast/stem cell growth factor receptor Kit (SCFR), Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, CD117 (c-Kit)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Properties

• biological source: rat
• Quality Level: 100
• conjugate: PE-Cy7
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: ACK2, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• UniProt accession no.: P05532
• target post-translational modification: unmodified
• Gene Information: mouse ... Kit(16590)

Description

General description

The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo. Please choose the appropriate format for each application. In addition, the antibody is reported to be cross-reactive with rat c-Kit and is extensively published for use in this species.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Anti-CD117 (c-Kit) (mouse), PE-Cy7, clone ACK2 Antibody is an antibody against CD117 for use in Flow Cytometry.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD117 (c-Kit) in one million C57Bl/6 bone marrow cells.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable