MABE1824

Anti-LbCpf1 Antibody, clone 2D5-6G11

clone 2D5-6G11, from mouse

• Synonym(s): Cpf1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2D5-6G11, monoclonal
• species reactivity: Lachnospiraceae, bacteria
• packaging: antibody small pack of 25 μg
• technique(s): western blot: suitable
• isotype: IgG1κ
• target post-translational modification: unmodified

Description

General description

CRISPR-associated endonuclease Cas12a (UniProt: A0A182DWE3; also known as CRISPR-associated endonuclease Cpf1; Lbcpf1) is a single crRNA-guided endonuclease of the class 2 CRISPR-Cas system that lacks tracrRNA and undergoes large conformational changes upon crRNA binding. Cpf1 has several features that can distinguish it from Cas9. Unlike Cas9 that uses dual-guide RNAs, Cpf1 is guided by a crRNA. It recognizes a T-rich PAM (T-rich protospacer adjacent motif), whereas Cas9 favors a G-rich PAM and Cpf1 generates staggered ends at its PAM-distal region, but Cas9 creates blunt ends at its PAM-proximal region. Cpf1 displays higher targeting specificity in mammalian cells when compared to Cas9. It has been shown that depending on the PAM sequences, LbCpf1 can undergo conformational changes to form altered interactions with the PAM-containing DNA duplexes, hence achieving the relaxed PAM recognition. (Ref.: Zetsche, B., et al. (2015). Cell 163(3); 759-77; Yamano, T., et al. (2017). Mol. Cell 67(4); 633-645; Gao, P., et al. (2016). Cell Res. 26(8); 901-913).

Specificity

Clone 2D5-6G11 specifically detects CRISPR-associated endonuclease Cas12a (LbCpf1).

Immunogen

Epitope: N-terminus

His-tagged recombinant fragment from the N-terminal region of Lachnospiracea bacteria.

Application

Anti-LbCpf1, clone 2D5-6G11 Antibody, Cat. No. MABE1824, is a highly specific mouse monoclonal antibody that targets LbCpf1 and has been tested for use in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: Various dilutions from a representative lot detected LbCpf1 in lysates from HEK293 cells expressing HA-tagged LbCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Quality

Evaluated by Western Blotting in HEK293 cells expressing HA-tagged LbCpf1.

Western Blotting Analysis: 1 µg/mL of this antibody detected LbCpf1 in lysate from HEK293 cells expressing HA-tagged LbCpf1.

Target description

~144 kDa observed; 143.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable