MAB5284
Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301
clone Cat-301, Chemicon®, from mouse
Synonym(s):
CSPG
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All Photos(8)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Cat-301, monoclonal
species reactivity
primate, human, rat, gerbil, feline, hamster, guinea pig
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACAN(176)
General description
The antigen recognized by this clone, CAT-301 is a proteoglycan that is developmentaly regulated, high molecular chondroitin sulphate proteoglycan found on the extracellular surface of mammalian neurons. It is expressed late in development and although no definitive role has been identified for CAT-301, it is believed to have a role in the stabilisation of synaptic structure.
The name derives from the name of the monoclonal antibody originally used to identified by its expression, which was detected immunocytochemicaly
Disruption of the normal patterns of neuronal activity during the critical early postnatal period by physical or biochemical means results in a large and irreversible reduction in levels of CAT-301. Similar intervention in mature animals has no effect
CAT-301 is related to aggrecan from articular cartilage. The CAT-301 antibody immunoreacts with aggrecan, and the chondroitinase treated aggrecan and CAT-301 both have apparent sizes of 550kDa. However CAT- 301 is not glycosylated by keratan sulphate, as aggrecan is.
The name derives from the name of the monoclonal antibody originally used to identified by its expression, which was detected immunocytochemicaly
Disruption of the normal patterns of neuronal activity during the critical early postnatal period by physical or biochemical means results in a large and irreversible reduction in levels of CAT-301. Similar intervention in mature animals has no effect
CAT-301 is related to aggrecan from articular cartilage. The CAT-301 antibody immunoreacts with aggrecan, and the chondroitinase treated aggrecan and CAT-301 both have apparent sizes of 550kDa. However CAT- 301 is not glycosylated by keratan sulphate, as aggrecan is.
Specificity
Chondroitin sulfate proteoglycan (CSPG), brain core protein.
Immunogen
Epitope: Brain (core protein)
Feline spinal cord fixed gray matter.
Application
Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301 detects level of Chondroitin Sulfate Proteoglycan & has been published & validated for use in IP, WB, IC, IH.
Immunohistochemistry: 1:500-1:2,000 on 4% paraformaldehyde fixed frozen tissue.
Immunocytochemistry
Immunoblot
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Immunocytochemistry
Immunoblot
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Differential expression of several molecules of the extracellular matrix in functionally and developmentally distinct regions of rat spinal cord.
Laura Vitellaro-Zuccarello,Paola Bosisio,Samanta Mazzetti,Claudia Monti,Silvia De Biasi
Cell and Tissue Research null
A surface antigen that identifies ocular dominance columns in the visual cortex and laminar features of the lateral geniculate nucleus.
Hockfield, S, et al.
Cold Spring Harbor Symposia on Quantitative Biology, 48 Pt 2, 877-889 (1983)
Histochemical differences between motoneurons supplying multiply and singly innervated extraocular muscle fibers.
Andreas C Eberhorn, Patricia Ardeleanu, Jean A Buttner-Ennever, Anja K E Horn
The Journal of Comparative Neurology null
A surface antigen expressed by a subset of neurons in the vertebrate central nervous system.
Hockfield, S and McKay, R D
Proceedings of the National Academy of Sciences of the USA, 80, 5758-5761 (1983)
A C Eberhorn et al.
Neuroscience, 137(3), 891-903 (2005-12-07)
In mammals, the extraocular muscle fibers can be categorized in singly-innervated and multiply-innervated muscle fibers. In the monkey oculomotor, trochlear and abducens nucleus the motoneurons of multiply-innervated muscle fibers lie separated from those innervating singly-innervated muscle fibers and show different
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