MAB5262

Anti-Neurofilament 200 kDa Antibody, clone RT97

clone RT97, Chemicon^®, from mouse

• Synonym(s): Anti-CMT2CC, Anti-NFH
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RT97, monoclonal
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_021076.2
• UniProt accession no.: P12036
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Description

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.

Specificity

The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).

Immunogen

Rat brain protein preparation

Application

Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.

Immunohistochemistry:
1-10 μg/mL

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Quality

Evaluated by Western Blot on Mouse brain membrane lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.

Target description

200 kDa

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Brain

Cultured neurons

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable