MAB363

Anti-NMDAR1 Antibody, clone 54.1

clone 54.1, Chemicon^®, from mouse

• Synonym(s): N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 54.1, monoclonal
• species reactivity: rat, monkey, human, Xenopus
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; radioimmunoassay: suitable
• isotype: IgG2a
• NCBI accession no.: NM_000832.5
• UniProt accession no.: Q05586
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: frog ... Grin1(780048) ; human ... GRIN1(2902) ; rat ... Grin1(24408) ; rhesus monkey ... Grin1(574380)

Description

General description

Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.

Specificity

Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.

Immunogen

Epitope: Extracellular loop between transmembrane regions III & IV.

Recombinant fusion protein containing GST and NMDAR1 aa. 660-811.

Application

Anti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.

Immunocytochemistry:
A previous lot of this antibody was used in IC.

RIA:
A previous lot of this antibody was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA.

Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.

Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by immunohistochemistry on rat brain and spinal cord tissue.

Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord

Target description

103-116 kDa

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat brain and spinal cord tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable