MAB3234
Anti-Cytokeratin 18 Antibody, clone RGE53
clone RGE53, Chemicon®, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RGE53, monoclonal
species reactivity
human, pig, mouse, chicken, canine, rat, rabbit, hamster
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... KRT18(3875)
General description
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Specificity
Specifically recognizes glandular epithelial cells from gastro-intestinal tract, respiratory tract and urogenetical tract. As well as endocrine and exocrine tissues and mesothelial cells. It is unreactive with stratified squamous epithelia (esophagus, epidermis, ectocervix). In transitional epithelium of the bladder RGE53 only reacts with the superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumours. The antibody is directed against 45 kD cytokeratin protein (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.
Immunogen
Cytoskeletal preparation from HeLa cells
Application
Anti-Cytokeratin 18 Antibody, clone RGE53 is an antibody against Cytokeratin 18 for use in FC, IC, IH & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Western blot
Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.
Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.
Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.
Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.
Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.
Optimal working dilutions must be determined by the end user.
Target description
45 kD
Linkage
Replaces: 04-586
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Skin , Pancreas/tumor
Skin , Pancreas/tumor
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
DeltaNp63 is essential for epidermal commitment of embryonic stem cells.
Alain Medawar,Thierry Virolle,Philippe Rostagno,Stephanie de la Forest-Divonne et al.
Testing null
Derivation and characterization of hepatocytes from embryonic stem cells in vitro.
Shigeaki Ishizaka,Yukiteru Ouji,Masahide Yoshikawa,Kazuki Nakatani
Methods in Molecular Biology null
Sandra S Essbauer et al.
Virology journal, 8, 339-339 (2011-07-07)
Approximately 60% of emerging viruses are of zoonotic origin, with three-fourths derived from wild animals. Many of these zoonotic diseases are transmitted by rodents with important information about their reservoir dynamics and pathogenesis missing. One main reason for the gap
V Ninichuk et al.
Kidney international, 70(1), 121-129 (2006-05-26)
Multipotent mesenchymal stem or stromal cells (MSC) have shown to improve outcome of acute renal injury models, but whether MSC can delay renal failure in chronic kidney disease is not known. We injected primary MSC or saline into mice that
Noushin Amirpour et al.
Advanced biomedical research, 3, 4-4 (2014-03-05)
Retinal pigment epithelium (RPE) is a hexagonal monolayer of pigmented cells located between the neural retina and the choroid with an essential role for visual function. So, isolation, propagation and maintenance of their functional integrity of RPE are crucial for
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service