MAB312RX
Anti-Neuron Cell Surface Antigen Antibody, clone A2B5-105 (7), Alexa Fluor™ 488
clone A2B5-105 (7), Chemicon®, from mouse
Synonym(s):
A2B5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A2B5-105 (7), monoclonal
species reactivity
mouse, chicken, human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
Specificity
The antibody recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:
· Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
· Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979.
· Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989].
· Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
· Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
· Human and rat thymic epithelial cells (Haynes, et al., 1988).
· Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & slerberg, 1989).
· Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
· Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)
· Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979.
· Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989].
· Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
· Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
· Human and rat thymic epithelial cells (Haynes, et al., 1988).
· Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & slerberg, 1989).
· Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
Immunogen
Embryonic chicken retinal cells
Application
Anti-Neuron Cell Surface Antigen Antibody, clone A2B5-105 (7), Alexa Fluor 488 detects level of Neuron Cell Surface Antigen & has been published & validated for use in IC, IH.
Immunocytochemistry
Immunohistochemistry: 1:30
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:30
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Physical form
Purified immunoglobulin conjugated to Alexa Fluor™ 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots in the dark for up to 6 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
The effect of TGF-beta2 on elastin, type VI collagen, and components of the proteolytic degradation system in human optic nerve astrocytes.
Neumann, Carolin, et al.
Investigative Ophthalmology & Visual Science, 49, 1464-1472 (2008)
Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells.
Bartholomeusz, R K, et al.
Endocrinology, 124, 2680-2685 (1989)
Flow cytometric analysis of rat striatal nerve terminals
Wolf, M E and Kapatos, G
The Journal of Neuroscience, 9, 94-105 (1989)
B F Haynes et al.
The Journal of clinical investigation, 71(1), 9-14 (1983-01-01)
Using a monoclonal antibody (A2B5), which binds to GQ ganglioside, and tetanus toxin, which binds to GD and GT gangliosides, distinct regions of human and rodent thymic epithelial cells have been identified. The lymphoid elements of the thymus do not
Bone marrow examination in neuroblastoma patients: a morphologic, immunocytochemical, and immunohistochemical study
Oppedal, B R, et al
Human Pathology, 20, 800-805 (1989)
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