MAB2056Z

Anti-Integrin α3 Antibody, clone ASC-1 (Azide Free)

clone ASC-1, Chemicon^®, from mouse

• Synonym(s): CD49c
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: ASC-1, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NM_002204.1; NM_005501.1
• UniProt accession no.: P26006
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGA3(3675)

Description

Specificity

Human alpha3 integrin (CD49c)

Immunogen

Human squamous cell carcinoma cells (SCC9)

Application

Inhibition (Functional Activity): A previous lot of this antibody was shown to inhibit cell attachment by inhibiting SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide and potentiates the inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. This antibody does not inhibit cell adhesion to fibronectin or collagen type IV.

Immunohistochemistry Analysis: A previous lot of this antibody was used on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

Flow Cytometry Analysis: A previous lot of this antibody stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).

Immunoprecipitation Analysis: A previous lot of this antibody precipitates PAGE bands of 130 kDa (reduced) or 116 kDa and 135 kDa (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.

Anti-Integrin α3 Antibody, clone ASC-1 (Azide Free) inhibits cell attachment & detects level of Integrin α3 & has been published & validated for use in FC, IP, IH, Inhibition Assays.

Research Category
Cell Structure

Research Sub Category
Integrins

Target description

116 kDa

Physical form

Format: Purified

Purified antibody in PBS without preservatives.

Storage and Stability

Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable