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Key Documents

MAB1276

Sigma-Aldrich

Anti-Nuclei & Chromosomes Antibody, histone H1 protein, clone 1415-1

clone 1415-1, Chemicon®, from mouse

Synonym(s):

Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1415-1, monoclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_005321.2

UniProt accession no.

P10412

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... H1-1(3024)

Specificity

Histone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts with preferentially with Histone H1 by ELISA and immunoblotting. MAB1276 produces a speckled nuclear staining pattern in all tissues studies.

Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse. Antibody reacts with rat cells {Bendeck, 2001}.

Immunogen

Epitope: histone H1 protein

Application

Anti-Nuclei & Chromosomes, histone H1 protein, clone 1415-1 is a high quality Mouse Monoclonal Antibody for the detection of Nuclei & Chromosomes & has been validated in ELISA, WB, ICC, IHC(P).

Physical form

100 μL Hybridoma Supernatant, concentrated by ammonium sulfate. Buffer: PBS with 0.1% sodium azide.
Format: Purified

Analysis Note

Control
POSITIVE CONTROL:

Human tonsil

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R Ikegami, J Zhang, A K Rivera-Bennetts, T D Yager
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Susanne Kaitna et al.
Current biology : CB, 12(10), 798-812 (2002-05-17)
Mitotic chromosome segregation depends on bi-orientation and capture of sister kinetochores by microtubules emanating from opposite spindle poles and the near synchronous loss of sister chromatid cohesion. During meiosis I, in contrast, sister kinetochores orient to the same pole, and
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T D Yager et al.
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