MAB1254B
Anti-Chymase Antibody, clone B7, biotin conjugated
clone B7, Chemicon®, from mouse
Synonym(s):
Mast Cell Protease I
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
culture supernatant
antibody product type
primary antibodies
clone
B7, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
dot blot: suitable
immunohistochemistry: suitable
isotype
IgG1κ
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CMA1(1215)
General description
Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
Specificity
Reacts with human mast cell chymase.
Immunogen
Chymase purified from human skin.
Application
Immunohistochemistry at 1:2,000:1:4,000.
For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.
Physical form
Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: lung, heart, skin, placenta.
POSITIVE CONTROL: lung, heart, skin, placenta.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J H McKerrow et al.
Molecular medicine (Cambridge, Mass.), 6(5), 450-460 (2000-08-22)
Proteases facilitate several steps in cancer progression. To identify proteases most suitable for drug targeting, actual enzyme activity and not messenger RNA levels or immunoassay of protein is the ideal assay readout. An automated microtiter plate assay format was modified
Highly increased numbers of leukocytes in inflamed gingiva from patients with HIV infection.
Maung Myint, Svein Steinsvoll, Zuanning N Yuan, Berit Johne, Kristen Helgeland, Karl Schenck
AIDS null
Phenotypic change and accumulation of smooth muscle cells in strictures in Crohn's disease: relevance to local angiotensin II system.
Takehisa Suekane,Yoshihiro Ikura,Kenji Watanabe,Junko Arimoto,Yoko Iwasa,Yoshimi Sugama et al.
Journal of Gastroenterology null
Fixation with Carnoy's fluid reduces the number of chymase-positive mast cells: not all chymase-positive mast cells are also positive for tryptase
KleinJan, A. et al.
Allergy, 51, 614-620 (1996)
Arnold S Kirshenbaum et al.
PloS one, 11(7), e0159177-e0159177 (2016-07-28)
Hermansky-Pudlak Syndrome type-1 (HPS-1) is an autosomal recessive disorder caused by mutations in HPS1 which result in reduced expression of the HPS-1 protein, defective lysosome-related organelle (LRO) transport and absence of platelet delta granules. Patients with HPS-1 exhibit oculocutaneous albinism
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