EZBRAIN42
Human Amyloid β42 ELISA Kit
measures and quantifies Amyloid β42 levels in 50 μL tissue or cell lysates
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.77
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product name
Human Amyloid β42 Brain ELISA, This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
Quality Level
species reactivity
human
packaging
kit of 1 × 96 wells
parameter
50 μL sample volume (Overnight assay)
assay range
sensitivity: 8 pg/mL
(lowest level of Amyloid β1-42 standard; 50 μL sample size)
standard curve range: 16-500 pg/mL
technique(s)
ELISA: suitable
input
sample type tissue/cellular lysate
application(s)
research use
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... APP(351)
General description
Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.
Specificity
The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Alzheimer′s Disease
Alzheimer′s Disease
This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
This assay requires 50 µl of sample and is an overnight assay.
Used to detect/quantify: Amyloid β42
Storage and Stability
Components in the kit can be stored up to 2 weeks at 2-8°C
Other Notes
Please contact Technical Service for linearity of dilution.
Disclaimer
For research use only. Not for use in diagnostic procedures.
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Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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