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ECM580

Sigma-Aldrich

QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric

The QCM Haptotaxis Cell Migration Assay, 24-well plate -Fibronectin, Colorimetric is ideal for the study of epithelial & fibroblast cell haptotaxis.

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

species reactivity

vertebrates

manufacturer/tradename

Chemicon®
QCM

technique(s)

cell based assay: suitable

detection method

colorimetric

shipped in

wet ice

General description

Also available: Cell Comb Scratch Assay! Get biochemical data from a scratch assay!Click Here

Introduction
The CHEMICON QCM Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric is ideal for the study of epithelial and fibroblast cell haptotaxis. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 8 μm pore size of this assay′s Boyden chambers is not appropriate for the study of lymphocyte migration.
• Each kit provides sufficient materials for the evaluation of 12 samples.
• Please refer to the product insert for assay details.

The CHEMICON QCM Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric assay is intended for research use only; not for diagnostic applications.

Application

Research Category
Cell Structure

Packaging

Sufficient for analysis of 12 samples

Components

Fibronectin Test Plate: (Part No. 2005689) One 24-well culture plate, containing 12 human FN-coated Boyden chambers, sufficient for the evaluation of 12 test samples.

BSA Control Plate: (Part No. 2005791) One 24-well culture plate, containing 12 BSA-coated Boyden chambers, sufficient for the evaluation of 12 controls.

Cell Stain Solution*: (Part No. 20294) One vial - 10 mL.

Extraction Buffer: (Part No. 20295) One vial - 10 mL.

24 well Stain Extraction Plate: (Part No. 2005871). One plate.

96 well Stain Quantitation Plate: (Part No. 2005870). One plate.

Swabs: (Part No. 10202) 50 each.

Forceps: (Part No. 10203) 1 pair

*Cell Stain Solution contains a small amount of crystal violet, which is toxic if swallowed or inhaled, and may cause irritation to the eyes, respiratory system, and skin. Handle with caution.

Linkage

Replaces: ECM500

Storage and Stability

Store at 4°C for up to one year after date of purchase.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thamara J Abouantoun et al.
Molecular cancer therapeutics, 8(5), 1137-1147 (2009-05-07)
Platelet-derived growth factor (PDGF) receptor (PDGFR) expression correlates with metastatic medulloblastoma. PDGF stimulation of medulloblastoma cells phosphorylates extracellular signal-regulated kinase (ERK) and promotes migration. We sought to determine whether blocking PDGFR activity effectively inhibits signaling required for medulloblastoma cell migration
Astrid Hensel et al.
iScience, 25(6), 104355-104355 (2022-05-24)
The unique threonine protease Tasp1 impacts not only ordered development and cell proliferation but also pathologies. However, its substrates and the underlying molecular mechanisms remain poorly understood. We demonstrate that the unconventional Myo1f is a Tasp1 substrate and unravel the

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