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Key Documents

AP1168

Sigma-Aldrich

Anti-TNFSF14 Mouse mAb (4E3)

liquid, clone 4E3, Calbiochem®

Synonym(s):

Anti-Tumor Necrosis Factor Receptor Like 2, Anti-Tumor Necrosis Factor Superfamily Member 14, Anti-Tumor Necrosis Factor Superfamily Member 14, Anti-Tumor Necrosis Factor Receptor Like 2

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4E3, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNFSF14(8740)

General description

Purified mouse monoclonal antibody. Recognizes the TNFSF14 protein.
Recognizes the TNFSF14 protein.
This Anti-TNFSF14 Mouse mAb (4E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of TNFSF14.

Immunogen

A recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein

Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human spleen tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mario C Manresa et al.
Gastroenterology, 159(5), 1778-1792 (2020-07-28)
Eosinophilic esophagitis (EoE) is an antigen-mediated eosinophilic disease of the esophagus that involves fibroblast activation and progression to fibrostenosis. Cytokines produced by T-helper type 2 cells and transforming growth factor beta 1 (TGFβ1) contribute to the development of EoE, but

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