ABS232
Anti-MIXL1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Homeobox protein MIXL1, Homeodomain protein MIX, hMix, MIX1 homeobox-like protein 1, Mix.1 homeobox-like protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
primate (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MIXL1(83881)
General description
MIXL1 is also known as Homeobox protein MIXL1, Homeodomain protein MIX (hMix), MIX1 homeobox-like protein 1, and Mix.1 homeobox-like protein. MIXL1 is a transcription factor with roles in axial mesendoderm morphogenesis, endoderm formation, blood cell differentiation, and morphogenesis of the heart and the gut during embryogenesis. MIXL1 has been shown to negatively regulate brachyury expression. MIXL1 is expressed mainly in progenitors and secondary lymph tissues, immature B- and T-lymphoid cells during normal hematopoiesis, and is also present in differentiating embryonic stem cells.
Specificity
Other homologies: Mouse and Rat (85% sequence homology).
This antibody recognizes the homeobox DNA binding domain of MIXL1.
Immunogen
Epitope: Homeobox DNA binding domain
KLH-conjugated linear peptide corresponding to the homeobox DNA binding domain of human MIXL1.
Application
Anti-MIXL1 Antibody is a highly specific rabbit polyclonal antibody, that targets Homeobox protein MIXL1 & has been tested in western blotting & ICC.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MIXL1 in H9 embryoid body cells. This protein was observed in H9 embryoid bodies (Lee, M. R., et al. (2010). Stem Cells. 28:1550-1559). MIXL1 was also localized in the nucleus of COS-7 and mouse ES cells (Mossman, A. K., et al. (2005). Stem Cells and Development. 14:656-663).
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MIXL1 in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MIXL1 in 10 µg of Jurkat cell lysate.
Target description
~36 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Man Ryul Lee et al.
Stem cells (Dayton, Ohio), 28(9), 1550-1559 (2010-07-29)
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