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Key Documents

ABE569

Sigma-Aldrich

Anti-monoubiquitin H2A Antibody (Lys119)

from rabbit, purified by affinity chromatography

Synonym(s):

H2AK119ub1, Histone H2A.1, Histone H2A.2, H2A2, h2aUb

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

XP_001021342

UniProt accession no.

P0C0S8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

rat ... Hist2H2Aa2(690131)

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The ubiquitination of Histones plays essential roles in the regulation of DNA related processes.

Immunogen

Branch peptide corresponding to human monoubiquitin H2A (Lys119).

Application

Detect Histone H2A using this rabbit polyclonal antibody, Anti-monoubiquitin H2A Antibody (Lys119) validated for use in western blotting, ChIP, IP and ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot from an independent laboratory detected monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected monoubiquitin H2A (Lys119) in 10 µg of HeLa acid extract.

Target description

~23 kDa observed. Uniprot describes one isoform at 14 kDa; however, this protein may be observed at ~23 kDa due to monoubiquitination.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 90 mM Tris-glycine pH8, 50 mM Nacl, and 10% glycerol with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
HeLa acid extract

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mei-Jun Zhao et al.
Oncotarget, 7(8), 8956-8967 (2016-01-21)
The ubiquitin ligase RNF8 promotes the DNA damage response (DDR). We observed that the expression of RNF8 was increased in bladder cancer cells and that this change in RNF8 expression could be reversed by adenovirus-mediated shRNA treatment. Moreover, we found
Barbara Dupret et al.
PloS one, 11(7), e0158700-e0158700 (2016-07-22)
Polycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of
Fei-Fei Hu et al.
Molecular therapy. Nucleic acids, 27, 670-684 (2022-01-25)
The disruption of epigenetic regulation is common in tumors; the abnormal expression of epigenetic factors leads to cancer occurrence and development. In this study, to investigate the potential function of histone methylation regulators in lung adenocarcinoma (LUAD), we performed differential
KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands.
Farcas, AM; Blackledge, NP; Sudbery, I; Long, HK; McGouran, JF; Rose, NR; Lee, S; Sims et al.
eLife null
Lan-Tao Gou et al.
Cell, 169(6), 1090-1104 (2017-05-30)
Genetic studies have elucidated critical roles of Piwi proteins in germline development in animals, but whether Piwi is an actual disease gene in human infertility remains unknown. We report germline mutations in human Piwi (Hiwi) in patients with azoospermia that prevent its ubiquitination
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