ABE347
Anti-NFκB p65 Antibody, CT
from rabbit
Synonym(s):
v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), Nuclear factor of kappa light polypeptide gene enhancer
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μg
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... RELA(5970)
General description
The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.
Specificity
This antibody recognizes NFκB at the C-terminus.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human NFκB.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-NFκB p65 Antibody, CT (Rabbit Polyclonal Antibody) validated in WB, IP to detect NFκB p65 also known as transcription factor p65.
Western Blot (SNAP i.d.) Analysis: 5 µg/mL from a representative lot detected NFκB on 10 µg of A431 cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated NFκB from A431 RIPA lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated NFκB from A431 RIPA lysate.
Quality
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NFκB in 10 µg of A431 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NFκB in 10 µg of A431 cell lysate.
Target description
~65 kDa observed.
Four isoforms produced by alternative splicing have been described: Isoform 1 (p65) at 60.2 kDa, Isoform 2 (p65 delta 2) at 58.8 kDa, Isoform 3 (p65 delta) at 59.1 kDa, and Isoform 4 at 59.9 kDa
Four isoforms produced by alternative splicing have been described: Isoform 1 (p65) at 60.2 kDa, Isoform 2 (p65 delta 2) at 58.8 kDa, Isoform 3 (p65 delta) at 59.1 kDa, and Isoform 4 at 59.9 kDa
Linkage
Replaces: 06-418
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
A431 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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