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Key Documents

ABC463

Sigma-Aldrich

Anti-Osteoprotegerin/TNFRSF11B Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_002537

UniProt accession no.

O00300

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TNFRSF11B(4982)

General description

Osteoprotegerin/TNFRSF11B, also known as Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, or Osteoprotegerin, (OPG), and encoded by the gene TNFRSF11B/OCIF/OPG, is a membrane bound receptor that acts as a decoy receptor for TNFSF11/RANKL and thereby helps to neutralize or regulate its function in osteoclastogenesis. Bone homeostasis is at least partially dependent upon the levels of TNFSF11 and TNFSF11B, and by acting as decoy or "extra" receptor for TNFSF11B the excess factor levels can be modulated. The activation of the receptors inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. OPG also seems to act as a decoy receptor for TNFSF10/TRAIL and help prevent against TRAIL mediated apoptosis, and TRAIL acts as antagonist for osteoclastogenesis, and thus again OPG appears to moderate osteoclast levels in a dynamic fashion. OPG is highly expressed in numerous tissues other than developing and adult bone germinal centers, but its exact role in these other tissues is still being examined.

Specificity

Immunogen is 92% identical in bovine and in mouse. Immunogen is 84% identical in rat.

Immunogen

Epitope: TNFR-Cys 2 domain
KLH-conjugated linear peptide corresponding to the TNFR-Cys 2 domain of human Osteoprotegerin/TNFRSF11B.

Application

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Osteoprotegerin/TNFRSF11B in human kidney tissue.
This Anti-Osteoprotegerin/TNFRSF11B Antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Osteoprotegerin/TNFRSF11B.

Quality

Evaluated by Western Blotting in MG63 conditioned media.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Osteoprotegerin/TNFRSF11B in 10 µg of MG63 conditioned media.

Target description

~55 kDa observed. Protein is heavily glycosylated and often observed as a monomer at ~55-60 kDa (Cundy T., et al. (2002) Hum Mol Genet. 11(18):2119-27).

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tim Cundy et al.
Human molecular genetics, 11(18), 2119-2127 (2002-08-22)
Idiopathic hyperphosphatasia is an autosomal recessive bone disease characterized by deformities of long bones, kyphosis and acetabular protrusion, increasing in severity as affected children pass through adolescence. Biochemical and histological evidence indicate that there is extremely rapid bone turnover, with
Chun-Sheng Bi et al.
Clinical and experimental dental research, 5(4), 377-388 (2020-01-17)
This study aimed to investigate the relationship between inflammation-related T-helper cell polarization and the receptor activator for nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) ratio, which is associated with bone resorption or remodeling of chronic periodontitis patients. Gingival crevicular fluid (GCF) and

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