AB5860-200UL

Anti-Potassium Channel TREK-1 Antibody

Chemicon^®, from rabbit

• Synonym(s): Twik-Related K+ Channel 1, Kcnk2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat
• species reactivity (predicted by homology): mouse (17/18)
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_001017424.2; NM_001017425.2; NM_014217.3
• UniProt accession no.: O95069
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... KCNK2(3776)

Description

Specificity

Other species have not been tested.

Recognizes TREK-1 (Twik-Related K+ Channel 1, Kcnk2). The epitope does not share homology with any other known proteins.

Immunogen

Synthetic peptide corresponding amino acids 8-28 of human TREK-1 (Accession numbers AAF89743, AAD47569).

Application

Detect Potassium Channel TREK-1 using this Anti-Potassium Channel TREK-1 Antibody validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1:200 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min.)

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3