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technique(s)
protein quantification: suitable
single molecule counting: suitable
suitability
suitable for immunoassay
detection method
fluorometric
General description
Single Molecule Counting (TM=["SMC"]) Technology: Reduced background + increased signal
TM=["SMC"] technology provides maximum immunoassay performance while following a workflow similar to traditional ELISA technology. By combining a unique assay elution step and robust digital counting, TM=["SMC"] technology achieves improved signal to noise ratios over traditional immunoassay technologies. The TM=["SMC"] technology thus provides enhanced quantification at both low and high levels of expression on one complete system.
TM=["SMC"] Assay Workflow
During the capture and detection steps, specific antibodies translate each biomarker into a signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The TM=["SMCxPRO"] system is the second generation instrument supporting TM=["SMC"] technology assays. The TM=["SMCxPRO"] technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).
Digital counting improves sensitivity and dynamic range
The TM=["SMCxPRO"] instrument captures the sum of all digital events counted above a background threshold. The TM=["SMCxPRO"] instrument digital event algorithm computes digital events across a spectrum of time series for a single standard curve, thus improving assay sensitivity
and extending the dynamic measuring range beyond what can be achieved with traditional technologies.
TM=["SMC"] technology provides maximum immunoassay performance while following a workflow similar to traditional ELISA technology. By combining a unique assay elution step and robust digital counting, TM=["SMC"] technology achieves improved signal to noise ratios over traditional immunoassay technologies. The TM=["SMC"] technology thus provides enhanced quantification at both low and high levels of expression on one complete system.
TM=["SMC"] Assay Workflow
During the capture and detection steps, specific antibodies translate each biomarker into a signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The TM=["SMCxPRO"] system is the second generation instrument supporting TM=["SMC"] technology assays. The TM=["SMCxPRO"] technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).
Digital counting improves sensitivity and dynamic range
The TM=["SMCxPRO"] instrument captures the sum of all digital events counted above a background threshold. The TM=["SMCxPRO"] instrument digital event algorithm computes digital events across a spectrum of time series for a single standard curve, thus improving assay sensitivity
and extending the dynamic measuring range beyond what can be achieved with traditional technologies.
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