688225

Caspase-1 Substrate VI, Fluorogenic

Fluorogenic caspase-1 substrate.

• Synonym(s): Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
• Empirical Formula (Hill Notation): C₃₃H₃₆F₃N₅O₁₀
• CAS Number: 219137-85-6
• Molecular Weight: 719.66
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: 98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white to off-white
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1
• InChI key: GWKJISJKNKIJNP-DEQDFGQZSA-N

Description

General description

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic caspase-1 substrate

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Tyr-Val-Ala-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable