567707

Sphingomyelinase, Staphylococcus aureus

• Synonym(s): Sphingomyelinase, Staphylococcus aureus
• CAS Number: 9031-54-3
• Enzyme Commission number: 3.1.4.12 (BRENDA, IUBMB)
• MDL number: MFCD00132362
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: Staphylococcus aureus
• Quality Level: 100
• form: liquid
• specific activity: ≥100 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native sphingomyelinase from Staphylococcus species. May be applied exogenously to initiate the sphingomyelin signaling pathway. Has very little activity on phosphatidylcholine.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of trinitrophenylaminolauryl (TNPAL)-sphingomyelin per min at 37°C, pH 7.4.

Physical form

In 250 mM phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial use, aliquot and refrigerate (4°C).

Other Notes

Galve-Roperh, I., et al. 1997. Brain Res. Mol. Brain Res.52, 90.
Jarvis, W.D., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 73.
Mathias, S., et al. 1993. Science259, 519.
Raines, M.A., et al. 1993. J. Biol. Chem.268, 14572.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2