524734
Phosphodiesterase 3B, His•Tag®, Human, Recombinant, S. frugiperda
Synonym(s):
PDE3B, cGMP-inhibited phosphodiesterase 3B
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
form
liquid
Quality Level
specific activity
≥0.5 U/μL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
General description
Full-length, recombinant, human phosphodiesterase 3B (accession no. AY459346) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. An important component of insulin and cAMP-dependent signaling. Supplied as a partially purified preparation.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.
Physical form
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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