Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

480066

Sigma-Aldrich

Necrostatin-1, Inactive Control

A cell-permeable N-demethylated thiohydantoin analog of Nec-1 that is devoid of anti-necroptotic properties and serves as a suitable inactive control.

Synonym(s):

Necrostatin-1, Inactive Control, 5-(Indol-3-ylmethyl)-2-thiohydantoin, Nec-1i

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C12H11N3OS
CAS Number:
64419-92-7
Molecular Weight:
245.30
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥95% (HPLC)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

light yellow

solubility

DMSO: 10 mg/mL
methanol: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C12H11N3OS/c16-11-10(14-12(17)15-11)5-7-6-13-9-4-2-1-3-8(7)9/h1-4,6,10,13H,5H2,(H2,14,15,16,17)

InChI key

MPLRRPKKFHUEEL-UHFFFAOYSA-N

General description

A cell-permeable N-demethylated thiohydantoin analog of Nec-1 (Cat. No. 480065) that is devoid of anti-necroptotic properties and serves as a suitable inactive control.

Note: This inactive inhibitor of necrosis should not be used in high concentration.
A cell-permeable N-demethylated thiohydantoin analog of Nec-1 (Cat. No. 480065) that is devoid of anti-necroptotic properties and serves as a suitable inactive control.



Note: This inactive inhibitor of necrosis should not be used in high concentration.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Inactive control for Nec-1 (Cat. No. 480065)
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Takahashi et al. 2012. Cell Death and Disease3, e437.
Degterev, A., et al. 2005. Nat. Chem. Biol.1, 112.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

N Takahashi et al.
Cell death & disease, 3, e437-e437 (2012-11-30)
Necrostatin-1 (Nec-1) is widely used in disease models to examine the contribution of receptor-interacting protein kinase (RIPK) 1 in cell death and inflammation. We studied three Nec-1 analogs: Nec-1, the active inhibitor of RIPK1, Nec-1 inactive (Nec-1i), its inactive variant
Alexei Degterev et al.
Nature chemical biology, 1(2), 112-119 (2006-01-13)
The mechanism of apoptosis has been extensively characterized over the past decade, but little is known about alternative forms of regulated cell death. Although stimulation of the Fas/TNFR receptor family triggers a canonical 'extrinsic' apoptosis pathway, we demonstrated that in

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service