475911

Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

• Synonym(s): Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
• CAS Number: 9003-99-0
• Enzyme Commission number: 1.11.1.7 (BRENDA, IUBMB)
• UNSPSC Code: 12352202

Properties

• biological source: human
• Quality Level: 200
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• specific activity: 150-200 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble (volume is lot-specific and can be found on the product label)
• shipped in: wet ice
• storage temp.: −20°C
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Description

General description

Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.

Physical form

Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

Preparation Note

Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.

Other Notes

Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3