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Sigma-Aldrich

Anti-KDEL Mouse mAb (10C3)

liquid, clone 10C3, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

10C3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Kdelr1(68137)

General description

Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Recognizes the Grp78 (BiP) and Grp94 proteins.
This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Immunogen

Rat
a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH

Application

Immunoblotting (4 µg/ml, colorimetric)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Highly Toxic (H)

Physical form

In PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells

Other Notes

Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael A Robichaux et al.
Disease models & mechanisms, 15(5) (2022-03-12)
The P23H mutation in rhodopsin (Rho), the rod visual pigment, is the most common allele associated with autosomal-dominant retinitis pigmentosa (adRP). The fate of misfolded mutant Rho in rod photoreceptors has yet to be elucidated. We generated a new mouse
Gary P Brennan et al.
PloS one, 8(1), e54491-e54491 (2013-01-30)
14-3-3 proteins are ubiquitous molecular chaperones that are abundantly expressed in the brain where they regulate cell functions including metabolism, the cell cycle and apoptosis. Brain levels of several 14-3-3 isoforms are altered in diseases of the nervous system, including
Tobias Becker et al.
iScience, 24(12), 103521-103521 (2021-12-18)
Protein AMPylation is a posttranslational modification with an emerging role in neurodevelopment. In metazoans two highly conserved protein AMP-transferases together with a diverse group of AMPylated proteins have been identified using chemical proteomics and biochemical techniques. However, the function of

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