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371720

Sigma-Aldrich

Anti-Giα-1-Subunit, Internal (159-168) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

mouse ... Gnai1(14677)

General description

Anti-Giα-1-Subunit, Internal (159-168), rabbit polyclonal, recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. It is validated for use in Western blotting.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-1 subunit.
Recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits.

Immunogen

a synthetic peptide (LDRIAQPNYI) corresponding to amino acids 159-168 of mouse Giα-1, conjugated to KLH

Application

Immunoblotting (1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

Serum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, Goα and Gsα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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