324402
Anti-Dopamine D₃ Receptor Rabbit pAb
lyophilized, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... DRD3(1814)
rat ... Drd3(29238)
General description
Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
Recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors.
Immunogen
a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
Application
ELISA (see comments)
Flow Cytometry (1:100, see application references)
Immunoblotting (1:500)
Immunohistochemistry (1:5000)
Flow Cytometry (1:100, see application references)
Immunoblotting (1:500)
Immunohistochemistry (1:5000)
Warning
Toxicity: Standard Handling (A)
Reconstitution
Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Other Notes
This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jacqueline S Coley et al.
PloS one, 10(2), e0117450-e0117450 (2015-02-04)
Drug abuse is a major comorbidity of HIV infection and cognitive disorders are often more severe in the drug abusing HIV infected population. CD14+CD16+ monocytes, a mature subpopulation of peripheral blood monocytes, are key mediators of HIV neuropathogenesis. Infected CD14+CD16+
Tina M Calderon et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 12(2), 353-370 (2017-01-31)
In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14+CD16+ monocytes mediate HIV entry into the central nervous system (CNS) and that
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