322326
Diphtheria Toxin, Unnicked, Corynebacterium diphtheriae
Diphtheria toxin catalyzes ADP-ribosylation of eukaryotic aminoacyltransferase II (EF2) using NAD as substrate. Activation requires nicking with a protease followed by reduction with DTT.
Synonym(s):
DTx
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About This Item
UNSPSC Code:
12352200
Recommended Products
Quality Level
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
2-8°C
Biochem/physiol Actions
Cell permeable: no
Note: Toxicity may vary by lot of toxin. Each laboratory should determine the optimum dosage for each particular application.
Primary Target
Eukaryotic aminoacyltransferase II (EF2)
Eukaryotic aminoacyltransferase II (EF2)
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Harmful (C)
Physical form
Lyophilized from sterile 10 mM Tris, 1 mM EDTA, pH 7.5.
Reconstitution
Following reconstitution, aliquot, quickly freeze on dry ice, and freeze (-70°C). Subsequent thawing should be carried out only at room temperature. For assays employing very low concentrations of diphtheria toxin, the use of a carrier protein, such as BSA or HSA, is recommended.
Please see vial label for lot-specific reconstitution volume.
Analysis Note
Major band (under reducing conditions) of ~63 kDa
Other Notes
Diphtheria toxin is purified from Corynebacterium diphtheriae Park Williams strain 8 by a modified method of Pappenheimer, et al. As assessed by disc electrophoresis run at alkaline pH under non-denaturing conditions, this preparation migrates as a major band at 63 kDa, corresponding to the intact toxin. Two faster, more lightly stained bands (24 and 39 kDa), corresponding to A and B fragments, may be observed. Following trypsin treatment in DTT, diphtheria toxin exhibits high activity when assayed for its ability to ADP-ribosylate EF2. The ED50 for CHO cells is determined to be about 0.4 ng/ml.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 1 Oral
Storage Class Code
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Tamar Licht et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(5), 974-995 (2020-01-22)
Multiple insults to the brain lead to neuronal cell death, thus raising the question to what extent can lost neurons be replenished by adult neurogenesis. Here we focused on the hippocampus and especially the dentate gyrus (DG), a vulnerable brain
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