234374

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli, is a pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells.

• Synonym(s): rmGM-CSF
• UNSPSC Code: 12352202

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• Assay: >97% (SDS-PAGE)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1.0 EU/μg Endotoxin (EU/μg GM-CSF)
• shipped in: ambient
• storage temp.: −70°C

Description

General description

Recombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells.

Recombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that stimulates the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells. Target cells include macrophages, granulocytes, and eosinophils. GM-CSF is produced by many cell types, including endothelial cells, fibroblasts, and T-lymphocytes. It can be extracted from most tissues in concentrations exceeding that of serum levels. While many cells produce GM-CSF constitutively, levels of this lymphokine increase rapidly in response to endotoxin, phorbol esters, and certain antigens.

Biochem/physiol Actions

ED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Other Notes

Anaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
Watanabe, S., et al. 1996. J. Biol. Chem.271, 12681.
Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
Gough, N.M., et al. 1985. EMBO J. 4, 645.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable