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LiChrospher® 100 RP-8 (5 µm) HPLC Columns

L x I.D. 125 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

Synonym(s):

TM=["LiChrospher"] C8 TM=["LICHROCART"] cartridge for HPLC

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrospher® 100 RP-8 (5 µm) LiChroCART™ 125-4, suitable for HPLC

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

LiChrospher®

feature

endcapped: no

extent of labeling

12.5% Carbon loading

parameter

250 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

12.5 cm × 4 mm

surface area

350 m2/g

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

Related Categories

General description

HPLC cartridge
LiChrospher® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 125-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application


  • Determination of dopamine, noradrenaline, and adrenaline in Krebs-Henseleit solution by liquid chromatography coupled with tandem mass spectrometry: This study showcases the use of LiChrospher® 100 RP-8 (5 µm) in a LICHROCART™ 125-4 setup for precise neurotransmitter measurement in biological samples, advancing understanding in neurochemistry and cardiovascular research. The column′s selectivity and resolution enhance the detection of these small molecules, pivotal for biochemical assays and physiological studies (Britto-Júnior et al., 2021).

Analysis Note

Pressure: ≤ 45 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.8 - 2.0

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

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