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Supelco

LiChrospher® RP-8 (5 µm) HPLC Columns

L x I.D. 150 mm x 4.6 mm, LiChroCART® HPLC cartridge, manuCART™ holder required for use

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrospher® RP-8 (5µm) LiChroCART® 150-4.6, suitable for HPLC

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

LiChrospher®

feature

endcapped: no

extent of labeling

12.5% Carbon loading

parameter

250 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

15 cm × 4.6 mm

surface area

350 m2/g

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

General description

HPLC cartidge
LiChrospher® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 150-4.6 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application

  • Stability studies of crude plant material of Bacopa monnieri and quantitative determination of bacopaside I and bacoside A by HPLC.: This study focuses on the stability of crude plant material from Bacopa monnieri, specifically analyzing the stability of its active constituents, bacopaside I and bacoside A. High-performance liquid chromatography (HPLC) using Purospher® RP-18 (5 µm) columns was employed for the quantitative determination. The findings highlighted the efficacy of Purospher® RP-18 columns in separating and quantifying these compounds with high precision, making it a valuable tool in analytical chemistry for phytochemical studies (Srivastava et al., 2012).

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.8 - 2.0

Legal Information

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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