1.50471

Purospher^® STAR RP-18 endcapped (3 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm Hibar^® RT HPLC Column

• Synonym(s): Purospher^® STAR RP-18 endcapped (3µm) Hibar^® RT 250-4.6
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Purospher^® STAR RP-18 endcapped (3 µm), L × I.D. 250 mm x 4.6 mm Hibar^® RT HPLC column
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Purospher^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 17% Carbon loading
• parameter: 400 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 25 cm × 4.6 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 3 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: reversed phase; supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

Purospher^® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher^® STAR RP-18 endcapped, 3 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher^® STAR RP-18 endcapped, 3 µm Hibar^® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.The recommended guard column is Purospher^® STAR RP-18 endcapped (5 µm) LiChroCART^™ 4-4 part number 1.50250.0001. For direct connection to a Hibar^® RT columns the Precolumn holder for 4-4 LiChroCART^™ cartridges, part number 1.51487.0001 can be used.Item 1.50471.1003 is a Validation kit containing 3 Purospher^® STAR RP-18 endcapped, 3 µm Hibar^® RT 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 280 bar
Theoretical Plates (N/m) (Anthracene): ≥ 120000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 6.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany