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Supelco

LiChrosorb® RP-8 (5 µm) HPLC Columns

L x I.D. 125 mm x 4 mm, Hibar® RT HPLC Column

Synonym(s):

TM=["LiChrosorb"] C8 HPLC column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrosorb® RP-8 (5 µm) Hibar® RT 125-4, suitable for HPLC

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

LiChrosorb®

feature

endcapped: no

parameter

400 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low)

column L × I.D.

12.5 cm × 4 mm

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

General description

HPLC column
LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar 125-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.

Application


  • Determination of amphotericin B, liposomal amphotericin B, and amphotericin B colloidal dispersion in plasma by high-performance liquid chromatography.: This study by Egger et al. focuses on the HPLC determination of various forms of amphotericin B in plasma, utilizing LiChrosorb® RP-8 as the stationary phase. This method ensures accurate monitoring of these antifungal agents in clinical settings (Egger et al., 2001).

  • Determination of peptide hydrophobicity parameters by reversed-phase high-performance liquid chromatography.: This research by Rothemund et al. used LiChrosorb® RP-8 to determine the hydrophobicity of various peptides, providing valuable insights for peptide characterization in biochemical research (Rothemund et al., 1994).

  • HPLC determination of doxorubicin, doxorubicinol and four aglycone metabolites in plasma of AIDS patients.: Beijnen et al. employed LiChrosorb® RP-8 in an HPLC method to quantify doxorubicin and its metabolites in plasma, offering a robust tool for therapeutic drug monitoring in AIDS patients (Beijnen et al., 1991).

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Toluene): ≥ 55000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.5 - 1.5

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany

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