1.50269

Purospher^® STAR Si (5 µm)

L × I.D. 250 mm x 4 mm LiChroCART^® HPLC cartridge, manuCART^™ holder required for use

• Synonym(s): Purospher^® STAR SI (5 µm) LiChroCART^® 250-4
• UNSPSC Code: 41115709

Properties

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L3
• product line: Purospher^®
• feature: endcapped: no
• packaging: 1 ea of
• parameter: 250 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 25 cm × 4 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: Silica bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH range: 2-7.5
• separation technique: hydrophilic interaction (HILIC); normal phase
• storage temp.: no temp limit

Description

General description

Purospher^® STAR Si endcapped, 5 µm HPLC columns offers highest separation efficiency for normal-phase chromatography of low molecular weight compounds soluble in organic solvents.Purospher^® STAR Si, 5 µm LiChroCART^® 250-4 is a cartridge column which requires a manuCART^™ cartridge column holder part number 1.51486.0001. The manuCART^™ can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher^® STAR RP-8 endcapped (5 µm) LiChroCART^® 4-4 part number 1.50249.0001. LiChroCART^® cartridge columns mounted with a manuCART^™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar. The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex samples allowing significant solvent savings and improved sensitivity.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used for the separation of aflatoxins, carotenoids, oils, fat-soluble vitamins, lipids, and fatty acids.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Use with Normal-phase or HILIC conditions (needs to be flushed to HILIC mobile phase)
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 65 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 65000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): 0.5 - 2.0

Legal Information

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany

MANUCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13