126593
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade
Synonym(s):
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade
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About This Item
Recommended Products
grade
RIA grade
Assay
≥98% (agarose gel electrophoresis)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤3.0 ash
≤5.0% moisture
color
off-white
solubility
0.15 M sodium chloride: 10 mg/mL
cation traces
heavy metals: ≤10 ppm
shipped in
ambient
storage temp.
2-8°C
General description
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.
Application
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
- to block tissue sections of placenta in immunohistochemical (IHC) analysis
- as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
- as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo
Biochem/physiol Actions
Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
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