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MilliporeSigma
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Key Documents

1.07882

Millipore

Standard I nutrient broth

for the cultivation of fastidious bacteria

Synonym(s):

Standard nutrient broth

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About This Item

UNSPSC Code:
41106213

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.5 (37 °C, 25 g/L in H2O, after autoclaving)

solubility

25 g/L

bulk density

720 kg/m3

application(s)

clinical
microbiology

suitability

Enterococcus spp.
Escherichia coli
Listeria spp.
Shigella spp.
Staphylococcus spp.
Streptococcus spp.

General description

for the cultivation of fastidious bacteria

Analysis Note

Appearance (clearness)clearAppearance (colour)yellowish-brownpH-value (25 °C)7.3 - 7.7Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))10 - 100Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))10 - 100Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))10 - 100Inoculum on reference medium (Staphylococcus epidermidis ATCC 12228 (WDCM 00036))10 - 100Inoculum on reference medium (Streptococcus pyogenes ATCC 12344)10 - 100Inoculum on reference medium (Streptococcus pneumoniae ATCC 6301)10 - 100Inoculum on reference medium (Listeria monocytogenes ATCC 19118)10 - 100Inoculum on reference medium (Shigella flexneri ATCC 12022 (WDCM 00126))10 - 100Inoculum on reference medium (Enterococcus faecalis ATCC 11700)10 - 100Growth (Escherichia coli ATCC 25922 (WDCM 00013))good to very goodGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034))good to very goodGrowth (Staphylococcus aureus ATCC 6538 (WDCM 00032))good to very goodGrowth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036))good to very goodGrowth (Streptococcus pyogenes ATCC 12344)good to very goodGrowth (Streptococcus pneumoniae ATCC 6301)good to very goodGrowth (Listeria monocytogenes ATCC 19118)good to very goodGrowth (Shigella flexneri ATCC 12022 (WDCM 00126))good to very goodGrowth (Enterococcus faecalis ATCC 11700)fair to goodIncubation: 24 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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