07-371

Anti-Histone H2B Antibody

Upstate^®, from rabbit

• Synonym(s): H2B, Histone H2B, H2B histone family, member Q, histone 2, H2be, histone cluster 2, H2be
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, chicken
• species reactivity (predicted by homology): yeast (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_778225
• UniProt accession no.: Q8N257
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: Saccharomyces cerevisiae ... Htb2(852284) ; human ... H2BC1(255626)

Description

General description

Histone H2B type 3-B (UniProt: Q8N257; also known as H2B type 12) is encoded by the HIST3H2BB gene (Gene ID: 128312) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.

Specificity

This rabbit polyclonal antibody detects Histone H2B in human and chicken. It targets an epitope within 9 amino acids from the C-terminus region.

Immunogen

KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B.

Application

Anti-Histone H2B Antibody, Cat. No. 07-371, is a highly specific rabbit polyclonal antibody that targets Histone H2B and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blotting Analysis: 1-2 µg/mL from a representative lot detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.

Quality

Evaluated by Western Blotting in Chicken Core Histone.

Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.

Target description

~17 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: MABE15

Physical form

Format: Purified

Protein A chromatography

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, with 0.1% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Acid extract from either sodium butyrate or colcemid treated HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1