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Key Documents

07-357

Sigma-Aldrich

Anti-phospho-eNOS/NOS III (Ser114) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, bovine

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_000603

UniProt accession no.

P29474

shipped in

dry ice

target post-translational modification

phosphorylation (pSer114)

Gene Information

bovine ... Nos3(287024)
human ... NOS3(4846)

General description

Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS

Specificity

eNOS/NOS III phosphorylated on serine 114

Immunogen

Synthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.

Application

Detect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Quality

routinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate

Target description

132 kDa

Physical form

0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%
Immunoaffinity Chromatography

Storage and Stability

1 year at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Functional protein network activation mapping reveals new potential molecular drug targets for poor prognosis pediatric BCP-ALL.
Accordi, Benedetta, et al.
Testing, 5, e13552-e13552 (2010)
Stuart A Ritchie et al.
The Biochemical journal, 426(1), 85-90 (2009-11-21)
Insulin stimulates endothelial NO (nitric oxide) synthesis via PKB (protein kinase B)/Akt-mediated phosphorylation and activation of eNOS (endothelial NO synthase) at Ser-1177. In previous studies, we have demonstrated that stimulation of eNOS phosphorylation at Ser-1177 may be required, yet is
Shiqin Xiong et al.
The Journal of clinical investigation, 130(7), 3684-3698 (2020-04-17)
Unchecked inflammation is a hallmark of inflammatory tissue injury in diseases such as acute respiratory distress syndrome (ARDS). Yet the mechanisms of inflammatory lung injury remain largely unknown. Here we showed that bacterial endotoxin lipopolysaccharide (LPS) and cecal ligation and

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