07-1590
Anti-phospho Histone H2A.X Antibody (Tyr142)
from rabbit, purified by affinity chromatography
Synonym(s):
Histone H2A.x, H2a/x
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
dot blot: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)
General description
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The phosphorylated form of H2A.X is thought to be invloved with DNA repair, possibly through reorganizing chromatin into a more open state and allowing the recruitment of proteins to repair DNA double stranded breaks.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human phospho Histone H2A.X phosphorylated at Tyr142.
Application
Anti-phospho Histone H2A.X Antibody (Tyr142) is a highly specific rabbit polyclonal antibody, that targets Histone H2A.X & has been tested in western blotting & Dot Blot.
Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H2A.X (Tyr142) peptides, but not phospho Histone H2A.X (Tyr142) peptides or other peptides corresponding to modified histones.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by Western Blotting in colcemid treated HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2A.X (Tyr142) in colcemid treated HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2A.X (Tyr142) in colcemid treated HeLa cell lysate.
Target description
~16 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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