07-068-I

Anti-Calcineurin A/PP2B catalytic unit Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat, mouse
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_058738
• UniProt accession no.: P20651
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PPP3CA(5530) ; mouse ... Ppp3Ca(19055) ; rat ... Ppp3Ca(24674)

Description

General description

Calcineurin/PP2B Aβ, also known as Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, and encoded by the gene PPP3CB/CALNA2/CALNB/CNA2, is a calcium dependent, calmodulin stimulated catalytic subunit protein phosphatase critical in the function of Calcineurin an important phosphatase governing immune responses such as T cell activation and the T cell immune response. Calcineurin is a heterodimeric protein consisting of a catalytic subunit Calcineurin A, which contains an active site dinuclear metal center, and a tightly associated, myristoylated, Ca(2+)-binding subunit, Calcineurin B. Calcineurin/PP2B Aβ is the beta isoform of the catalytic active site of the subunit A of Calcineurin. Calcineurin also plays pivotal roles in signaling transduction cascades in a variety of other immune cells as well as non-immune cells like myocytes and neurons.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to rat Calcineurin A/PP2B catalytic unit near the C-terminus.

Application

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated Calcineurin A/PP2B catalytic unit in 500 µg of mouse brain tissue lysate.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

This Anti-Calcineurin A/PP2B catalytic unit antibody is validated for use in western blotting & IP for the detection of Calcineurin A/PP2B catalytic unit.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Calcineurin A/PP2B catalytic unit in 10 µg of human brain tissue lysate.

Target description

~57 kDa observed

Linkage

Replaces: 07-068

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable