05-623-AF488

Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor^™ 488 Conjugate

clone CTD4H8, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CTD4H8, monoclonal
• species reactivity: human, mouse
• species reactivity (predicted by homology): yeast (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NP_000928.1
• UniProt accession no.: P24928
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... POLR2A(5430) ; mouse ... Polr2A(20020) ; rat ... Polr2A(363633)

Description

General description

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.

Target description

~210-220 kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable