04-1565

Anti-NSD1 Antibody, clone 1NW-1A10

ascites fluid, clone 1NW-1A10, from mouse

• Synonym(s): Androgen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, NR-binding SET domain-containing protein, Nuclear receptor-binding SET domain-containing protein 1, Sotos syndrome, nuclear receptor binding SET domain protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1NW-1A10, monoclonal
• species reactivity: rat
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_032765.3
• UniProt accession no.: O88491
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Nsd1(18193) ; rat ... Nsd1(306764)

Description

General description

NSD1 (also known as KMT3B) is a histone methyltransferase shown to methylate lysine 36 of histone H3 and lysine 20 of histone H4. NSD1 is a described as an intermediary transcription factor and depending on its cellular context, it is able to either negatively or positively influence transcription. Expression of this gene has been described in skeletal muscles, kidneys, adult and fetal brain, thymus and spleen. Heterozygous mutations and deletions of the NSD1 gene are associated with Sotos Syndrome, an autosomal dominant condition characterized by macrocephaly, tall stature, facial elongation, accelerated bone aging, and mental retardation.

Specificity

This antibody recognizes NSD1.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to mouse NSD1.

Application

Anti-NSD1 Antibody, clone 1NW-1A10 is a mouse monoclonal antibody for detection of NSD1, also known as &rogen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Chromatin Biology

Used for immunohistochemistry on paraffin sections in Visser et al., (2012).
Used for fluorescent immunocytochemistry in Huang et al.,(1998).

Quality

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected NSD1 on 10 µg of PC12 cell lysate.

Target description

~ 300 kDa

Physical form

Unpurified

Unpurified mouse monoclonal IgG1κ in preservative-free ascites.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PC12 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable