03-0136-00

SMC^® Mouse IL-4 Discovery Immunoassay Kit

• Synonym(s): mouse IL 4 immunoassay kit, mouse IL-4 Single Molecule Counting Kit, mouse IL-4 ultra-sensitive immunoassay, mouse Interleukin 4 immunoassay kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• species reactivity: mouse
• Quality Level: 200
• manufacturer/tradename: SMC^®
• assay range: accuracy: 80-120%; (minimum of 10 samples spiked with acceptable recovery)
• technique(s): ELISA: suitable; single molecule counting: suitable

Description

General description

Interleukin-4 (IL-4) is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.

The SMC^® Mouse IL-4 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The Single Molecule Counting (SMC^®) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.

Used to detect/quantify:IL-4

Components

Assay Buffer
Mouse IL-4 Coating Antibody
Assay Plate
10X PBS
Blocking Buffer
Standard Diluent
Mouse IL-4 Detection Antibody
Mouse IL-4 Standard
SMC^® Mouse IL-4 Immunoassay Kit Instructions
10X Wash Buffer
Buffer C
Elution Buffer B

Analysis Note

Low-end CV% Range: 0-20%|Low-end CV% Average: 4%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H317,H319,H360FD,H412
• Precautionary Statements: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects