Orchid Culture Media Formulations
Orchid tissue culture has its own unique requirements. Below is formulation information for a number of media that are used to successfully culture Orchidaceae.
K4003 - Knudson C modified orchid medium
P0931 - Phytamax orchid maintenance medium without charcoal
P1056 - Phytamax orchid medium with charcoal and banana powder
P6668 - Phytamax orchid maintenance medium
P6793 - Phytamax orchid multiplication medium
M5524 - Murashige and Skoog basal salt mixture (MS)
| Component (mg/L) | K4003 | P0931 | P1056 | P6668 | P6793 | M5524* |
|---|---|---|---|---|---|---|
| Aluminum chloride • 6H2O | ||||||
| Ammonium nitrate | 825.0 | 825.0 | 825.0 | 825.0 | 1650.0 | |
| Ammonium phosphate monobasic | ||||||
| Ammonium sulfate | 500.0 | |||||
| Boric acid | 0.056 | 3.1 | 3.1 | 3.1 | 3.1 | 6.2 |
| Calcium chloride anhydrous | 166.0 | 166.0 | 166.0 | 166.0 | 332.2 | |
| Calcium nitrate | 694.4 | |||||
| Calcium phosphate tribasic | ||||||
| Cobalt chloride • 6H2O | 0.0125 | 0.0125 | 0.0125 | 0.0125 | 0.025 | |
| Cupric sulfate • 5H2O | 0.0624 | 0.0125 | 0.0125 | 0.0125 | 0.0125 | 0.025 |
| Na2-EDTA | 37.24 | 37.24 | 37.24 | 37.24 | 37.26 | |
| ZnNa2-EDTA | ||||||
| Ferric chloride • 6H2O | ||||||
| Ferric citrate | ||||||
| Ferric sulfate | ||||||
| Ferric tartrate • 2H2O | ||||||
| Ferrous sulfate • 7H2O | 25.0 | 27.85 | 27.85 | 27.85 | 27.85 | 27.8 |
| Magnesium sulfate | 122.125 | 90.35 | 90.35 | 90.35 | 90.35 | 180.7 |
| Manganese chloride • 4H2O | ||||||
| Manganese sulfate • H2O | 5.682 | 8.45 | 8.45 | 8.45 | 8.45 | 16.9 |
| Molybdenum trioxide | 0.016 | |||||
| Molybdic acid (sodium salt) • 2H2O | 0.125 | 0.125 | 0.125 | 0.125 | 0.25 | |
| Nickel chloride • 6H2O | ||||||
| Nickel sulfate • 6H2O | ||||||
| Potassium chloride | ||||||
| Potassium iodide | 0.415 | 0.415 | 0.415 | 0.415 | 0.83 | |
| Potassium nitrate | 950.0 | 950.0 | 950.0 | 950.0 | 1900.0 | |
| Potassium phosphate monobasic | 250.0 | 85.0 | 85.0 | 85.0 | 85.0 | 170.0 |
| Potassium sulfate | ||||||
| Sodium nitrate | ||||||
| Sodium phosphate monobasic | ||||||
| Sodium sulfate | ||||||
| Zinc nitrate • 6H2O | ||||||
| Zinc sulfate • 7H2O | 0.331 | 5.3 | 5.3 | 5.3 | 5.3 | 8.6 |
| Organics (mg/L) | K4003 | P0931 | P1056 | P6668 | P6793 | M5524* |
|---|---|---|---|---|---|---|
| Activated charcoal | 2000.0 | 2000.0 | ||||
| Adenine hemisulfate | ||||||
| Agar | ||||||
| Banana powder (50% malto-dextrin) | 30,000 | |||||
| 6-Benzylaminopurine (BA) | 2.0 | |||||
| D-Biotin | ||||||
| 6gamma-gamma- Dimethylallylaminopurine | ||||||
| Glycine (free base) | ||||||
| Indole-3-acetic acid | ||||||
| Indole-3-butyric acid | ||||||
| Kinetin | ||||||
| MES (free acid) | 1000.0 | 1000.0 | 1000.0 | 1000.0 | ||
| myo-Inositol | 100.0 | 100.0 | 100.0 | 100.0 | ||
| alpha-Naphthaleneacetic acid | 0.5 | |||||
| Nictotinic acid (free acid) | 1.0 | 1.0 | 1.0 | 0.5 | ||
| Peptone | 2000.0 | 2000.0 | 2000.0 | 2000.0 | ||
| Pyridoxine • HCl | 1.0 | 1.0 | 1.0 | 0.5 | ||
| Sucrose | 20,000 | 20,000 | 20,000 | 20,000 | 20,000 | |
| Thiamine • HCl | 10.0 | 10.0 | 10.0 | 1.0 |
*Composition for M5524, Murashige and Skoog Basal Salt Mixture, listed for purposes of comparison only.
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