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column	Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U)
column temp.	30 °C
mobile phase	[A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85)
flow rate	0.2 mL/min
pressure	1150 psi (79 bar)
sample	10 ng/mL in mobile phase
injection	1 µL
detector	MS, ESI(+), MRM mode

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Analysis Note	The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis^® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation.
Categories	Analytical Chromatography, Immunosuppressants
Featured Industry	Vitamins, Nutraceuticals, and Natural Products
suitability	application for HPLC

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